Thermo Fisher Scientific Avi Tag Monoclonal Antibody (1D11D10)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

-

Immunocytochemistry (ICC/IF)

1-3 µg/mL

-

Flow Cytometry (Flow)

1-3 µg 1 x 10^6 cells

-

ELISA (ELISA)

0.02-0.05 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1D11D10

Immunogen

Avi peptide conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.

Target Information

The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.