Thermo Fisher Scientific NWSHPQFEK Tag Monoclonal Antibody (5A9F9), HRP, THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

-

ELISA (ELISA)

0.001-0.002 µg/mL

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5A9F9

Immunogen

NWSHPQFEK peptide conjugated to KLH

Conjugate

HRP HRP HRP

View additional formats

• Unconjugated
• Biotin
• FITC
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 10mg/mL BSA

Contains

0.01% thimerosal

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin. GenScript THE™ NWSHPQFEK Tag Antibody [HRP], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody (A01732) conjugated with Horseradish peroxidase (HRP). The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK Tagged fusion proteins.

Target Information

The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.