Thermo Fisher Scientific NWSHPQFEK Tag Monoclonal Antibody (5A9F9), THE
Strep-tag II 단백질 분석용 모노클로날 항체로, 높은 특이성과 친화력을 가집니다. Western blot, ICC/IF, Flow cytometry, ELISA, Immunoprecipitation 등 다양한 응용에 적합합니다. Lyophilized 형태로 제공되며 Protein A로 정제되어 안정적입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–3 µg/mL |
| Flow Cytometry (Flow) | 1 µg/mL |
| ELISA | 0.001–0.002 µg/mL |
| Immunoprecipitation (IP) | 1–5 µg/mL |
| Miscellaneous (PubMed) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A9F9 |
| Immunogen | NWSHPQFEK peptide conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It is widely used in molecular biology protocols due to its strong affinity for biotin.
NWSHPQFEK (Strep-tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
NWSHPQFEK Tag Antibody is a useful tool for analysis and affinity purification of Strep-tag II fusion proteins.
Target Information
The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions.
The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin, allowing simple purification of proteins using affinity columns but limited to the C-terminus of recombinant proteins.
To enable N-terminal tagging, it was re-engineered and re-named Strep-tag II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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