Thermo Fisher Scientific NWSHPQFEK Tag Monoclonal Antibody (5A9F9), THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

-

Immunocytochemistry (ICC/IF)

1-3 µg/mL

-

Flow Cytometry (Flow)

1 µg/mL

-

ELISA (ELISA)

0.001-0.002 µg/mL

-

Immunoprecipitation (IP)

1-5 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5A9F9

Immunogen

NWSHPQFEK peptide conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• HRP
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins.

Target Information

The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.