Thermo Fisher Scientific UNG Polyclonal Antibody
Thermo Fisher Scientific의 UNG Polyclonal Antibody는 인간, 마우스, 토끼, 랫트 반응성을 가지며 WB, IHC, ICC/IF에 적합합니다. Protein G로 정제된 액상 형태로 제공되며, UNG1/UNG2 단백질 연구에 활용됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:10–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues C(281) R H F S K T N E L L Q K S G K K P(298) of human UNG1 (NP_003353.1). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2288290 |
Product Specific Information
This sequence also corresponds to residues 290–307 of human UNG2.
Suggested positive control: antigen standard for UNG (transient overexpression lysate), HeLa whole cell extract.
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters (PA and PB).
The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively), thus their mRNAs are not resolved as two species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity.
A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues.
The level and expression pattern of UNG1 and UNG2 differ between cell and tissue types. Additionally, the expression of UNG is cell cycle regulated, being higher in proliferating compared to non-proliferating tissues and cells.
UNG1 is a 304 amino acid protein.
UNG2 is a 313 amino acid protein.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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