Thermo Fisher Scientific Histone H2A.X Polyclonal Antibody
Histone H2A.X 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ELISA, ChIP 등 다양한 응용에 적합합니다. Human, Mouse, Sheep에 반응하며, Protein G로 정제된 액상 형태로 제공됩니다. DNA 손상 연구 및 히스톤 변형 분석에 활용 가능합니다.
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Thermo Fisher Scientific Histone H2A.X Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 5 µg/mL | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | 1:2,000 | - |
| ChIP assay | 1.5 µg per million cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Sheep |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mixture of synthetic peptides containing residues 1–15 and 81–96 of Human Histone H2A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075628 |
Product Specific Information
Suggested positive control: Jurkat or PBMC.
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. In eukaryotes, DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the carboxy terminus of H2AX, resulting in gamma-H2AX. This phosphorylation can be detected by Western blotting or immunofluorescence to assess DSB frequency.
Phosphatidylinositol 3-kinases such as ATM, DNA-PK, and ATR are implicated in H2AX phosphorylation. Structurally, H2A.X contains 143 amino acids. It is synthesized in both G1 and S-phase, with mRNA variants possessing or lacking polyA tails. The gene maps to chromosome 11q23.2–q23.3 in humans.
Histone H2A.X plays a key role in chromatin structure and DNA repair, regulating the interaction of MDC1 (mediator of DNA damage checkpoint protein 1) at sites of DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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