Thermo Fisher Scientific Phospho-Bcl-2 (Ser70) Polyclonal Antibody
인간 Bcl-2 단백질의 인산화된 Ser70 부위를 인식하는 rabbit polyclonal antibody. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제됨. PBS 기반 완충액에 BSA 및 glycerol 포함, 단기 4℃, 장기 -20℃ 보관 권장.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Human Bcl-2 (phospho Ser70). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.17 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736353 |
Product Specific Information
- Positive Control: Jurkat (1 µM paclitaxel treatment for 16 hr)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. Members of the BCL-2 family can form dimers to modulate apoptosis. For example, Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect. Bax and Bak promote cytochrome c release from mitochondria, initiating apoptosis. Constitutive expression of BCL-2, such as through translocation to the Ig heavy chain locus, is implicated in follicular lymphoma. Overexpression of BCL-2 has been linked to human cancers including B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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