Thermo Fisher Scientific CD4 Monoclonal Antibody (OTI8H3), TrueMAB
CD4 단백질을 인식하는 mouse monoclonal antibody로, Western blot과 IHC(P) 분석에 적합합니다. Human 시료에 반응하며, carrier-free 형태로 제공되어 다양한 결합 실험에 활용 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8H3 |
| Immunogen | Full length human recombinant protein of human CD4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD4 antigen is a co-receptor for MHC class II molecule recognition and HIV binding. It is primarily expressed on T helper cells and other immune cells such as monocytes, macrophages, and dendritic cells.
Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut.
CD4 assists in T-cell activation via interaction with the T-cell receptor complex and Lck kinase, and may mediate neuronal damage in immune-related CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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