Thermo Fisher Scientific CD4 Monoclonal Antibody (OTI6B7), TrueMAB
인간 CD4 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, Flow Cytometry에 사용 가능. 비결합형 형태로 제공되며, 고순도 친화 크로마토그래피 정제. 연구용으로 HIV 및 면역 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6B7 |
| Immunogen | Full length human recombinant protein of human CD4 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. It is mainly expressed in T helper cells and other immune cells including monocytes, macrophages, and dendritic cells.
At the tissue level, CD4 is found in thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut.
CD4 is crucial for early T-cell activation via interaction with the T-cell receptor complex and Lck kinase, and may mediate neuronal damage in infectious and immune-related CNS diseases.
Multiple alternatively spliced transcripts have been reported (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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