Thermo Fisher Scientific GPR27 Polyclonal Antibody
Rabbit polyclonal antibody targeting human GPR27, suitable for IHC and ELISA. High species homology across primates and mammals. Purified by antigen affinity chromatography, supplied as unconjugated liquid form. Ideal for GPCR research applications.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 10 µg/mL
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 18 amino acid peptide from internal region of human GPR27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550283 |
Product Specific Information
Percent identity with other species by BLAST analysis:
- Human, Gorilla, Marmoset, Elephant, Panda (100%)
- Mouse, Rat (94%)
- Opossum, Platypus (89%)
- Chicken, Xenopus (83%)
For IHC(P), use proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
G protein coupled receptors (GPCRs) are a large family of receptors characterized by seven transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters, hormones, or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins (G proteins).
The 375 amino acid GPCR GPR27 protein contains features typical of the GPCR family, including:
- Seven transmembrane domains
- Potential N-linked glycosylation sites in the N-terminal extracellular domain
- Conserved cysteine residues forming a disulfide bridge in the first and second extracellular loops
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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