Thermo Fisher Scientific Phospho-Chk1 (Ser345) Recombinant Rabbit Monoclonal Antibody (HL122)

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:3,000

Product Specifications

Product Specific Information

• Store as concentrated solution.
• Centrifuge briefly prior to opening vial.
• For short-term storage (1–2 weeks), store at 4°C.
• For long-term storage, aliquot and store at -20°C or below.
• Avoid multiple freeze-thaw cycles.

Target Information

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised.
CHK1 is a key mediator in the DNA damage-induced checkpoint network and functions to ensure genomic integrity upon genotoxic stress.
When the G2 or S checkpoint is abrogated by inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and apoptosis, while normal cells remain arrested in the G1 phase.
Thus, CHK1 inhibitors can enhance the efficacy of DNA-damaging agents in cancer therapy, making Chk1 an attractive therapeutic target, particularly in p53-deficient cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.