Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), PE, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.015 µg)/test
• Tested on normal human peripheral blood cells
• View 12 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 755
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation

Product Specific Information

The 5C3 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein belonging to the TNFR family. CD40 is expressed on B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. It regulates B cell development and maturation, induces Ig isotype-switching, and prevents apoptosis in combination with IL-4. The CD40–CD154 interaction is crucial for T-B cell communication and immune regulation.
5C3 is commonly used for B cell activation in in vitro assays.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for use at 5 µL (0.015 µg) per test
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD40 is a single-chain glycoprotein of the TNF/NGF receptor superfamily, showing homology to CD30. It is expressed on all B cells (except plasma cells), some epithelial cells, carcinomas, and lymphoid dendritic cells. CD40 is essential for immune and inflammatory responses, including:

• T cell-dependent immunoglobulin class switching
• Memory B cell development
• Germinal center formation

The transcription factor AKNA regulates CD40 expression, influencing cell interactions. CD40 interaction with TNFR2 mediates signal transduction. The CD40–CD154 pathway is implicated in amyloid-β-induced microglial activation, suggesting a role in Alzheimer’s disease. Two alternatively spliced transcript variants of CD40 are known.
Associated diseases: Type 3 Hyper-IgM immunodeficiency, CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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