Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), PE, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 5 publications

Functional Assay (Functional)

• Tested Dilution: –
• Publications: View 1 publication

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 610
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.

Applications Reported:
The HIP1 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
This HIP1 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets.
Use at 5 µL (0.5 µg) per test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test.

Excitation/Emission:
488–561 nm / 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Lasers
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD42b is a platelet activation marker involved in coagulation as an aggregating factor. It interacts with extracellular matrix and adhesion molecules. CD42b expression and activation are regulated by VEGF and PDGF. Together with GPIb beta, GPIX, and GPV, it forms the GPIb-IX-V receptor complex critical for platelet-rich thrombus formation. CD42b binds to von Willebrand factor (VWF) at vascular injury sites, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1, and P-selectin. The extracellular domain also contributes to metastasis. CD42b antigen is expressed on platelets and megakaryocytes in bone marrow. Several polymorphisms and mutations in CD42b are associated with Bernard-Soulier syndromes and platelet-type von Willebrand disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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