Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Super Bright 436, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.06 µg)/test
• View 16 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 710 / 725
• NovaFluor Yellow 660 / 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG with an apparent molecular weight of 50–80 kDa.
CD16 includes two genes:

• CD16A (FcγRIIIA): expressed in macrophages and NK cells as a transmembrane form
• CD16B (FcγRIIIB): expressed in neutrophils as a GPI-anchored form

Two polymorphisms (NA-1 and NA-2) exist for CD16B. Individuals homozygous for NA-2 show lower phagocytic capacity than NA-1. CD16 binds IgG immune complexes, showing preferential binding to IgG1 and IgG3 isotypes. Upon IgG binding, both isoforms trigger signaling cascades leading to ADCC, phagocytosis, degranulation, and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.06 µg) per test.
A test is defined as the amount of antibody staining cells in a final 100 µL volume. Recommended cell number: 10⁵–10⁸ cells/test.

Fluorochrome Information:

• Excitation: 405 nm
• Emission: 436 nm
• Recommended filter: 450/50 bandpass or equivalent

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD16 encodes a receptor for the Fc portion of IgG, involved in immune complex clearance, antibody-dependent cellular cytotoxicity (ADCC), and modulation of viral infection susceptibility.
The FCGR3A gene (CD16A) is expressed on NK cells as a transmembrane glycoprotein, while FCGR3B (CD16B) is expressed on neutrophils with a phosphatidylinositol linkage.
Mutations in FCGR3A are associated with immunodeficiency 20 and increased susceptibility to herpes zoster and systemic lupus erythematosus.
Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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