Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Super Bright 436, eBioscience
인간 및 비인간 영장류 CD16(FcγRIII) 검출용 3G8 단클론 항체. Super Bright 436 형광체 결합으로 405nm 레이저에 최적화. 유세포 분석(Flow Cytometry)에 사용 가능하며, 고순도 친화 크로마토그래피 정제. 연구용으로만 사용.
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Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Super Bright 436, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Baboon, Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G8 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2716985 |
Additional Formats Available
- APC-eFluor 780
- Functional Grade
- NovaFluor Blue 510 / 530 / 585 / 610-70S / 660-120S / 660-40S / 760
- NovaFluor Red 685 / 700 / 725 / 755
- NovaFluor Yellow 590 / 610 / 660 / 690 / 700 / 730 / 755
- PerCP-eFluor 710
- Super Bright 600 / 645 / 702
- Request custom conjugation
Product Specific Information
This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (low-affinity Fc gamma RIII). CD16 exists as two isoforms: FcγRIIIA (on monocytes, macrophages, NK cells) and FcγRIIIB (on neutrophils). Expression patterns vary among primates.
Binding of IgG activates signal transduction pathways leading to antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.
- Recognizes the same epitope as CB16; distinct from B73.1 clone.
- Reported for flow cytometric analysis.
- Tested at 5 µL (0.06 µg) per test (100 µL final volume).
- Recommended cell number: 10⁵–10⁸ cells/test.
- Excitation: 405 nm; Emission: 436 nm (use 450/50 bandpass filter).
- Compatible with violet laser.
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright conjugates to minimize non-specific interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD16 encodes a receptor that binds the Fc portion of IgG and mediates immune complex clearance, ADCC, and other immune functions. The gene FCGR3A is similar to FCGR3B, both located on chromosome 1.
- FCGR3A: Expressed on NK cells, membrane-anchored via transmembrane peptide
- FCGR3B: Expressed on neutrophils, anchored via phosphatidylinositol linkage
- Associated disorders: Immunodeficiency 20, Herpes Zoster, recurrent viral infections, systemic lupus erythematosus, alloimmune neonatal neutropenia
- Alternative splicing produces multiple isoforms
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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