Thermo Fisher Scientific Bim Polyclonal Antibody
Human Bim 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB와 IHC(P) 분석에 적합합니다. 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태입니다. 인간, 마우스, 랫에서 반응 가능성이 있으며, 세포 사멸 연구에 활용됩니다.
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Thermo Fisher Scientific Bim Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 22–40 of human Bim |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2065031 |
Product Specific Information
- Predicted to react with mouse and rat based on sequence homology.
- Detects a band of ~22 kDa in K562 and A549 whole cell lysates.
- Rabbit anti-Human Bim antibody recognizes human Bcl-2 interacting mediator of cell death (Bim), also known as Bod.
Target Information
BIM belongs to the BCL-2 protein family. Members of this family form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities.
The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3) and interacts with BCL2, BCL2L1/BCL-X(L), and MCL1, functioning as an apoptotic activator.
Expression of this gene can be induced by nerve growth factor (NGF) and the forkhead transcription factor FKHR-L1, suggesting a role in neuronal and lymphocyte apoptosis.
Transgenic mouse studies indicate that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection.
Several alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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