Thermo Fisher Scientific Phospho-GluR1 (Ser831) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000
• Publications: -

Product Specifications

Product Specific Information

• Predicted to react with chicken, dog, human, and mouse based on sequence homology.
• For detection of phosphoproteins, add serine phosphatase inhibitors (e.g., 10 mM NaF) to the sample buffer.
• Avoid milk or casein-based blocking solutions, as casein is a phosphoprotein and may cause high background.
• Detects a ~100 kDa band in rat brain lysates.
• Recognizes glutamate receptor 1 (GluR1), also known as AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, and GluA1, when phosphorylated at serine 831.

Target Information

GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of L-glutamate induces a conformational change that opens the cation channel, converting a chemical signal into an electrical impulse. The receptor rapidly desensitizes into a transient inactive state characterized by a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8, GluR1 shows resensitization, characterized by delayed accumulation of current flux upon continued glutamate application.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.