
Thermo Fisher Scientific TRPC7 (extracellular) Polyclonal Antibody
TRPC7 단백질의 세포외 루프를 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 적합. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)DQHVQDDTLHNVS, corresponding to amino acid residues 504–516 of human TRPC7, 2nd extracellular loop |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on sample size.
- The antibody ships as a lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For longer storage, aliquot and keep at -20°C.
- Avoid multiple freeze/thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
TRPC7 is a receptor-activated, non-selective calcium-permeant cation channel. It likely operates via a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC7 is activated by diacylglycerol (DAG) and may also respond to intracellular calcium store depletion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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