Thermo Fisher Scientific PRMT4 Monoclonal Antibody (3H2)
CARM1 단백질을 검출하는 Thermo Fisher PRMT4 단클론 항체(3H2). Western blot, IHC, ICC, Flow cytometry, ELISA, ChIP 등 다양한 응용에 적합. Human, Mouse, Rat 등 반응. 고순도 재조합 CARM1 항원 기반, 연구용 전용.
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Thermo Fisher Scientific PRMT4 Monoclonal Antibody (3H2)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
| ChIP assay | 4 µL/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H2 |
| Immunogen | Purified recombinant fragment of human CARM1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11154323 |
Product Specific Information
MA5-15796 targets CARM1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Non-human primate, Mouse, and Rat samples.
The immunogen is a purified recombinant fragment of human CARM1 expressed in E. coli.
MA5-15796 detects CARM1, which has a predicted molecular weight of approximately 65 kDa.
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine.
Protein arginine methylation is involved in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation.
Tissue Specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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