Thermo Fisher Scientific CXCL13 Polyclonal Antibody
마우스 CXCL13 단백질을 인식하는 염소 유래 폴리클로날 항체로, Western blot, IHC, ELISA 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. PBS 및 5% 트레할로스 완충액에 재구성 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL | View 1 publication |
| ELISA | - | View 1 publication |
| Neutralization (Neu) | 20–50 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant mouse CXCL13/BLC/BCA-1 (Ile22–Ala109) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610214 |
Product Specific Information
- In direct ELISAs, less than 1% cross-reactivity with recombinant human CXCL13 and GCP-2 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of antibody by the LAL method.
Target Information
CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in secondary lymphoid organs. It promotes migration of B lymphocytes (B-1 and B-2) to follicles of the spleen, lymph nodes, and Peyer’s patches.
Produced mainly by macrophages, including large peritoneal macrophages, Kupffer cells, and lung macrophages (CD11c+ and CD11c−).
The cellular ligand for CXCL13 is CXCR5 (also known as BLR-1), expressed on B cells, Tfh cells, and some memory T cells.
CXCL13 is implicated in chronic lymphocytic leukemia (CCL) and serves as a plasma biomarker for germinal center activity and broadly neutralizing antibody generation against HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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