Thermo Fisher Scientific Involucrin (Squamous Cell Terminal Differentiation Marker) Monoclonal Antibody (IVRN/827)
인볼루크린 단백질을 인식하는 인간 반응성 마우스 모노클론 항체로, IHC, ICC/IF, Flow Cytometry에 사용 가능. 단층상피 세포의 말단 분화 마커로 연구용에 적합. 200 µg/mL 농도의 액상 형태로 PBS 기반 버퍼에 보존.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IVRN/827 |
| Immunogen | Purified involucrin from human keratinocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Involucrin is a component of the keratinocyte crosslinked envelope, located in the cytoplasm and crosslinked to membrane proteins by transglutaminase. It is expressed in stratified squamous epithelia such as the cornea. In normal epidermis, involucrin is first expressed in the upper spinous layers, and in keratinocyte cultures, it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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