Thermo Fisher Scientific CD16 Monoclonal Antibody (MEM-154)
CD16+ 인간 과립구 및 NK 세포 인식용 단클론 항체로, CD16-158V/F 다형성 분석에 적합. WB, Flow, IP 등 다양한 응용 가능. Unconjugated 형태로 제공되며, 1 mg/mL 농도, PBS(pH 7.4) 저장. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 2 publications |
| Flow Cytometry (Flow) | 5–10 µg/mL | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Functional Assay | Assay-dependent | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-154 |
| Immunogen | Human granulocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1071341 |
Additional Formats
Product Specific Information
This antibody reacts with CD16+ granulocytes and can be used for mapping CD16-158V/F polymorphism on NK cells, requiring presence of V at amino acid 158.
- Western Blot: Non-reducing conditions
- Functional Application: Blocking
Target Information
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in clearance of immune complexes and functions such as cellular mediated cytotoxicity and enhancement of virus infections.
The gene FCGR3A shares high similarity with FCGR3B, located on chromosome 1.
- FCGR3A: Expressed on NK cells (integral membrane glycoprotein via transmembrane peptide)
- FCGR3B: Expressed on polymorphonuclear neutrophils (anchored via phosphatidylinositol linkage)
Mutations in FCGR3A are associated with:
- Immunodeficiency 20
- Susceptibility to recurrent viral infections
- Systemic lupus erythematosus
- Alloimmune neonatal neutropenia
Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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