Thermo Fisher Scientific IkB epsilon Polyclonal Antibody
Thermo Fisher Scientific의 IkB epsilon Polyclonal Antibody는 인간 NFKBIE에 특이적인 항체로 Western blot, IHC, ICC/IF에 사용됩니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공되어 안정적인 신호 검출에 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NFKBIE. Recombinant protein control fragment (Product #RP-99392). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789386 |
Product Specific Information
Immunogen sequence:
AEESQYDSGI ESLRSLRSLP ESTSAPASGP SDGSPQPCTH PPGPVKEPQE KEDADGERAD STYGSSSLTY TLSLLGGPEA EDPAPRLPLP HVGALSPQQL EALTYISEDG DTLVHLAVIH EAPAVLLCCL ALLPQEVLDI Q
Target Information
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5'-GGGRNNYYCC-3' or 5'-HGGARNYYCC-3'. For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1, and NFAT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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