Thermo Fisher Scientific Catalase Polyclonal Antibody, Biotin
Catalase 단백질을 인식하는 Biotin 결합 Rabbit Polyclonal 항체로, Western blot, ELISA, Immunoprecipitation 등에 사용 가능. Bovine liver 유래 immunogen으로 제작되었으며, 고순도 Ion-exchange chromatography로 정제. 연구용으로 안정성과 재현성이 우수함.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:10,000–1:40,000 |
| Immunoprecipitation (IP) | 1:500–1:2,500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Catalase (Bovine Liver) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Anti-Catalase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, and purified Catalase (Bovine Liver).
Cross-reactivity against Catalase from other sources may occur but has not been specifically determined.
Target Information
Catalase is a homotetrameric heme-containing enzyme present within peroxisomes. It converts hydrogen peroxide into water and oxygen via dismutation reaction.
Human catalase includes the C-terminal sequence (-KANL) for peroxisome targeting and has a molecular weight of approximately 61.3 kDa per monomer.
Catalase is implicated in inflammation, mutagenesis, apoptosis prevention, and tumor stimulation.
Loss of catalase activity results in the genetic disease acatalasemia (Takahara’s disease).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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