Thermo Fisher Scientific Glutamate Dehydrogenase Polyclonal Antibody, Biotin

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunoprecipitation (IP)

• Tested Dilution: 1:500–1:2,000

Dot Blot (DB)

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by multi-step processes including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, and purified Glutamate Dehydrogenase (Bovine Liver).
Cross-reactivity with Glutamate Dehydrogenase from other species may occur but has not been determined.

Target Information

Glutamate dehydrogenase (GluD1) is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.
It plays a key role in regulating amino acid-induced insulin secretion and is allosterically activated by ADP and inhibited by GTP and ATP.
Activating mutations in this gene are a common cause of congenital hyperinsulinism.
Alternative splicing results in multiple transcript variants.
A related gene, Glutamate Dehydrogenase 2, located on the human X chromosome, encodes a soluble form of the enzyme.
Related pseudogenes are found on chromosomes 10, 18, and X.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.