Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), eBioscience
Thermo Fisher CD47 Monoclonal Antibody (miap301)는 마우스 CD47 단백질을 특이적으로 인식하는 Rat IgG2a 형 항체로, Flow Cytometry, Western blot, IHC(F), IP 등에 사용됩니다. 고순도(>90%)로 제조되었으며, PBS buffer에 0.09% sodium azide를 포함하고 4°C에서 보관합니다.
- 카탈로그번호
- 14-0471-8x (3개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 0.5 µg/test | View 3 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | miap301 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_837144 |
Available Formats:
- APC (17-0471-82)
- FITC (11-0471-82)
- Functional Grade (16-0471-81)
- PE-Cyanine7 (25-0471-80)
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody miap301 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin. CD47 is a glycosylated five-transmembrane protein with a small cytoplasmic domain involved in adhesion through interactions with SIRP and non-covalently associated with β3 integrins (CD51/CD61, CD41/CD61). It also serves as a receptor for thrombospondin.
- No cross-reactivity is observed against human CD47.
- Expression is found in most hematopoietic cells (T cells, B cells, monocytes, platelets, erythrocytes) and non-hematopoietic cells.
- Reported applications include Flow Cytometry, Immunoprecipitation, Western blot (nonreducing conditions), and Immunohistology of frozen tissue sections.
Tested Application Example:
- Flow Cytometric analysis of mouse splenocytes.
- Recommended ≤ 0.5 µg/test (100 µL final volume).
- Cell number range: 10⁵–10⁸ cells/test.
- Antibody titration recommended for optimal assay performance.
Purity & Quality:
- Purity: >90% (SDS-PAGE)
- Aggregation: <10% (HPLC)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD47 (Integrin-Associated Protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with integrins and regulates their functions. Engagement of CD47 modulates signaling pathways such as activation of G proteins and intracellular calcium increase during cell adhesion. CD47 is a receptor for thrombospondin and plays roles in membrane transport and signal transduction.
- Broad tissue distribution; reduced on Rh erythrocytes.
- Acts as a ligand for CD172a (SIRPα), inhibiting macrophage phagocytosis of CD47-positive cells.
- Involved in cell migration, B cell adhesion, T cell activation, chondrocyte mechanical response modulation, and neuronal development—especially abundant in synapse-rich regions of brain and retina.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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