Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV (395, 496, 563, 615, 737)
• Brilliant Violet (421, 605, 650, 711)
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor (Blue, Red, UV, Violet, Yellow series)
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright (436, 600, 645, 702, 780)

Product Specific Information

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

Applications Reported:
Flow cytometric analysis, immunoprecipitation, immunohistochemical staining, immunoblotting (non-reducing conditions), and complement-dependent cytotoxicity.
For functional assays, use Functional Grade purified IM7 (Product #16-0441).

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. Recommended ≤0.125 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize concentration for specific assay conditions.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD44 is a 100 kDa type I transmembrane glycoprotein expressed on human leukocytes, brain white matter, and epithelial cells of intestine and breast. Multiple isoforms exist, with CD44H being predominant in normal tissues. It acts as a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, adhesion, and migration. CD44 participates in lymphocyte activation, recirculation, and homing, and may be upregulated in carcinomas with potential links to metastasis. Expression is found in hematopoietic, non-hematopoietic, and epithelial cells, as well as filopodia in cultured keratinocytes. Alternative splicing produces diverse isoforms. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.