Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), eBioscience
CD44 단백질을 인식하는 IM7 클론의 Rat monoclonal antibody로, Human 및 Mouse 시료에 반응합니다. Flow cytometry, Western blot, IHC 등 다양한 응용에 사용 가능하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. Affinity chromatography로 정제되었으며 4°C에서 보관합니다.
- 카탈로그번호
- 14-0441-8x (4개 옵션)
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 5 |
| Immunohistochemistry (IHC) | Assay-Dependent | 22 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | 1:500 | 18 |
| Flow Cytometry (Flow) | 0.125 µg/test | 344 |
| ELISA | - | 1 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 1 |
| Functional Assay | Assay-Dependent | 3 |
| Miscellaneous PubMed | - | 4 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Fruit fly, Horse, Human, Mouse, Non-human primate |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IM7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467245 |
Available Conjugated Formats
- Alexa Fluor 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395, 496, 563, 615, 737)
- Brilliant Violet (421, 605, 650, 711)
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor (Blue, Red, UV, Violet, Yellow series)
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright (436, 600, 645, 702, 780)
Product Specific Information
The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.
Applications Reported:
Flow cytometric analysis, immunoprecipitation, immunohistochemical staining, immunoblotting (non-reducing conditions), and complement-dependent cytotoxicity.
For functional assays, use Functional Grade purified IM7 (Product #16-0441).
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. Recommended ≤0.125 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize concentration for specific assay conditions.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD44 is a 100 kDa type I transmembrane glycoprotein expressed on human leukocytes, brain white matter, and epithelial cells of intestine and breast. Multiple isoforms exist, with CD44H being predominant in normal tissues. It acts as a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, adhesion, and migration. CD44 participates in lymphocyte activation, recirculation, and homing, and may be upregulated in carcinomas with potential links to metastasis. Expression is found in hematopoietic, non-hematopoietic, and epithelial cells, as well as filopodia in cultured keratinocytes. Alternative splicing produces diverse isoforms. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.