Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience

Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

Alexa Fluor 488, 532, 561, 660, 700
APC, APC-eFluor 780, Biotin
Brilliant UV/Violet series (395–805)
eFluor 450–660, FITC, PE, PE-Cyanine series
PerCP-Cyanine5.5, PerCP-eFluor 710
Super Bright series (436–780)
NovaFluor Blue, Red, Violet, Yellow series
Functional Grade available
Custom conjugation upon request

Product Specific Information

Description:
The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. Some activated T cells, LAK cells, NK progenitors, and T cells of lpr/lpr mutant mice also express this antigen.

Applications Reported:
Suitable for flow cytometric analysis, immunoprecipitation, and immunohistochemical staining (frozen or formalin-fixed paraffin sections). Also used in functional B cell studies. For functional assays, use Functional Grade RA3-6B2 (Product #16-0452).

Applications Tested:

• Flow cytometry: ≤1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
• IHC (Paraffin): ≤5 µg/mL
• IHC (Frozen): ≤5 µg/mL
  Careful titration recommended for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD45R (PTPRC) is a protein tyrosine phosphatase crucial for hematopoietic cell signaling, regulating T- and B-cell receptor activation. It contains extracellular, transmembrane, and cytoplasmic catalytic domains. CD45 isoforms arise from alternative splicing and differ in extracellular regions. These isoforms influence membrane localization and expression patterns depending on cell type and physiological state. CD45 also modulates integrin-mediated signaling and apoptosis-related DNA fragmentation.
Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.