Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience
Mouse CD45R/B220 항원을 인식하는 RA3-6B2 클론의 Rat 모노클론 항체. Flow cytometry, IHC, IP 등 다양한 응용에 적합하며, B 세포 계열 분석에 사용. Unconjugated 포맷으로 제공되며 높은 순도(>90%)와 안정적 저장 조건을 가짐.
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- 14-0452-8x (4개 옵션)
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- pk
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Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 71 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | 9 publications |
| Immunocytochemistry (ICC/IF) | - | 12 publications |
| Flow Cytometry (Flow) | 1 µg/test | 255 publications |
| ELISA | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 1 publication |
| Miscellaneous (PubMed) | - | 2 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Fish, Human, Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RA3-6B2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467253 |
Available Conjugated Formats
Alexa Fluor 488, 532, 561, 660, 700
APC, APC-eFluor 780, Biotin
Brilliant UV/Violet series (395–805)
eFluor 450–660, FITC, PE, PE-Cyanine series
PerCP-Cyanine5.5, PerCP-eFluor 710
Super Bright series (436–780)
NovaFluor Blue, Red, Violet, Yellow series
Functional Grade available
Custom conjugation upon request
Product Specific Information
Description:
The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. Some activated T cells, LAK cells, NK progenitors, and T cells of lpr/lpr mutant mice also express this antigen.
Applications Reported:
Suitable for flow cytometric analysis, immunoprecipitation, and immunohistochemical staining (frozen or formalin-fixed paraffin sections). Also used in functional B cell studies. For functional assays, use Functional Grade RA3-6B2 (Product #16-0452).
Applications Tested:
- Flow cytometry: ≤1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
- IHC (Paraffin): ≤5 µg/mL
- IHC (Frozen): ≤5 µg/mL
Careful titration recommended for optimal assay performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45R (PTPRC) is a protein tyrosine phosphatase crucial for hematopoietic cell signaling, regulating T- and B-cell receptor activation. It contains extracellular, transmembrane, and cytoplasmic catalytic domains. CD45 isoforms arise from alternative splicing and differ in extracellular regions. These isoforms influence membrane localization and expression patterns depending on cell type and physiological state. CD45 also modulates integrin-mediated signaling and apoptosis-related DNA fragmentation.
Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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