Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), PE-Cyanine5, eBioscience

Applications and Tested Dilution

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Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 711
• Brilliant Violet 786
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The IT2.2 monoclonal antibody reacts with human CD86 (B7-2), an ~80 kDa surface receptor. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated upon stimulation via BCR, CD40, or cytokine receptors. CD86 interacts with CD28 and CTLA-4 on T cells, playing a key role in T-B cell communication, T cell costimulation, and immune regulation.

Applications Reported:
This antibody is validated for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test in a final volume of 100 µL. Cell number: 10⁵–10⁸ cells/test (empirically determined).

Light Sensitivity:
PE-Cyanine5 is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation/Emission:
488–561 nm / 667 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD86 is a ligand for CTLA-4 and CD28, acting as a costimulatory molecule in T-cell activation. Insufficient co-stimulation can induce tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed on antigen-presenting cells. It interacts with CD28 to activate T-cells and with CTLA-4 to inhibit activation. Alternative splicing produces multiple transcript variants. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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