Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine5, eBioscience
마우스 CD80(B7-1) 인식 단클론 항체로 Flow Cytometry에 최적화된 PE-Cyanine5 형광 접합체입니다. 0.06 µg/test로 사용 가능하며, 고순도 Affinity chromatography 정제. 488–561 nm 여기, 667 nm 방출 특성으로 다양한 레이저에 호환됩니다.
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Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine5, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.06 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Mouse, Pig |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16-10A1 |
| Conjugate | PE-Cyanine5 |
| Excitation / Emission Max | 568 / 666 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468773 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. It binds with high affinity to CD28 and CD152 (CTLA-4) on T cells, playing a crucial role in T-B cell communication and activation.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on activated mouse splenocytes at ≤0.06 µg/test (100 µL final volume). Cell count may vary (10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.
Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation: Samples can be fixed using IC Fixation Buffer (100 µL sample + 100 µL buffer) or 1-step Fix/Lyse Solution for up to 3 days at 4°C in the dark with minimal brightness loss.
Excitation/Emission: 488–561 nm / 667 nm; compatible with Blue, Green, and Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells and various lymphoma cell lines. Dysfunction in CD80 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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