Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), PE-Cyanine5, eBioscience
인간 CD90(Thy-1) 인식 단클론 항체로, PE-Cyanine5 형광체 결합형입니다. Flow cytometry에 최적화되어 있으며, 5 µL/test로 사용됩니다. 인간 및 마우스 반응성을 가지며, 광민감성 주의가 필요합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), PE-Cyanine5
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 47 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio5E10 (5E10) |
| Conjugate | PE-Cyanine5 |
| Excitation / Emission Max | 568 / 666 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10669699 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 786
- FITC
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Request custom conjugation available
Product Specific Information
The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25–35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, fetal liver cells, cord blood cells, and bone marrow cells. It is involved in regulation of adhesion and signal transduction by T cells.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested on human erythroleukemia (HEL) cells; use at 5 µL (0.25 µg) per test (100 µL final volume)
- Cell number range: 10⁵–10⁸ cells/test
- Light sensitivity: Tandem dye is sensitive to photo-induced oxidation; protect from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark
- Excitation: 488–561 nm; Emission: 667 nm; Laser: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90 (Thy-1) antigen is a GPI-linked glycoprotein of the Immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer cells, and fibroblasts. CD90 plays a role in cell-cell or cell-ligand interactions during synaptogenesis and other neural events. It serves as a marker for various stem cells and axonal processes of mature neurons. Structural studies of CD90 contributed to defining the Immunoglobulin superfamily and the first biochemical characterization of a vertebrate GPI anchor.
Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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