Thermo Fisher Scientific O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (HGAC85)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA1-076 recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins.
It has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and ELISA procedures.
By Western blot, this antibody detects several proteins representing immunoprecipitated O-GlcNAc glycoproteins.
Immunofluorescence staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties.
The immunogen is heat killed, pepsin treated group A streptococci.

Note: Do not use with diluents containing glycosylated proteins.

Target Information

O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues.
Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm.
They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral, and transcription regulatory proteins.
O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.