Thermo Fisher Scientific Hepatitis C Virus Core Antigen Monoclonal Antibody (C7-50)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 44 publications 44 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 57 publications 57 publications

Flow Cytometry (Flow)

1/100

View 4 publications 4 publications

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C7-50

Immunogen

Purified HCV core-GST fusion protein (genotype 1b).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325417

Product Specific Information

MA1-080 detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines.

MA1-080 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures. By Western blot, this antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines. Immunofluorescence staining of HCV core protein in transfected chimp hepatocytes yields a staining pattern consistent with cytoplasmic and vesicular staining.

The MA1-080 immunogen is purified HCV core-GST fusion protein (genotype 1b). This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains.

Target Information

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.