Thermo Fisher Scientific CaV3.2 Monoclonal Antibody (N55/10)
CaV3.2 단백질을 검출하기 위한 Mouse IgG1 단일클론 항체로, WB, IHC, ICC 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성을 가지며, Protein G 정제 후 PBS/glycerol buffer에 보관됩니다. 고특이성으로 Cav3.2를 검출하며 Cav1.3과 교차 반응하지 않습니다.
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Thermo Fisher Scientific CaV3.2 Monoclonal Antibody (N55/10)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N55/10 |
| Immunogen | Fusion protein amino acids 1019–1293 (II–III loop) of human Cav3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735142 |
Available Conjugated Formats
Product Specific Information
1 µg/mL of MA5-27656 was sufficient for detection of Cav3.2 in 10 µg of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 260 kDa. No cross-reactivity against Cav1.3.
Target Information
This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell.
Multiple isoforms exist, encoded by different genes or generated by alternative splicing. Certain mutations in this gene have been associated with childhood absence epilepsy (CAE).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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