Thermo Fisher Scientific ID3 Monoclonal Antibody (OTI5C7), TrueMAB
ID3 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI5C7). Western blot, IHC, ICC/IF, Flow cytometry에 적합. Human 시료 반응성. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C7 |
| Immunogen | Full length human recombinant protein of human ID3 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting column: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The ID family of basic helix-loop-helix (bHLH) proteins includes ID1, ID2, ID3, and ID4.
These proteins are ubiquitously expressed and form heterodimers with class A and B HLH proteins.
Due to the lack of a basic region, these heterodimers cannot bind DNA, thus ID proteins negatively regulate DNA binding of bHLH proteins.
ID1 inhibits DNA binding of E12 and Myo D, thereby suppressing muscle-specific gene expression.
During muscle cell differentiation, ID protein levels decrease, allowing E12/E47 to interact with Myo D and myogenin to activate myogenic differentiation.
Expression of ID proteins is growth factor-dependent, and reduction of ID mRNA delays cell cycle reentry following growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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