Thermo Fisher Scientific PTS Polyclonal Antibody
Western blot, IHC, ICC에 사용 가능한 Rabbit Polyclonal 항체로 Human 및 Mouse에 반응. 항원 친화 크로마토그래피로 정제되었으며 1 mg/mL 농도의 액상 형태. BH4 생합성 관련 PTS 단백질 타깃. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length human PTS recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11157847 |
Product Specific Information
- Recommended positive controls: 293T, H1299, HeLa, mouse stomach, PTS-transfected 293T
- Predicted reactivity: Mouse (86%), Rat (87%), Rhesus Monkey (97%), Bovine (84%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The tetrahydrobiopterin (BH4) cofactor is essential for hepatic hydroxylase, which is involved in phenylalanine degradation and catecholamine and serotonin biosynthesis. BH4 is also an essential and limiting cofactor for all types of nitric oxide synthase. BH4 deficiency results in hyperphenylalaninemia and monoamine neurotransmitter depletion and is most commonly due to autosomal recessive mutation in 6-pyruvoyltetrahydropterin synthase (PTPS), the second enzyme for BH4 biosynthesis.
The active site of PTPS consists of the pterin-anchoring Glu A107 neighbored by two catalytic motifs: a Zn(II) binding site and an inter-subunit catalytic triad formed by Cys A42, Asp B88 and His B89. The active site of PTPS undergoes a Zn and Mg-dependent reaction that includes a triphosphate elimination, a stereospecific reduction and the oxidation of both side hydroxyl groups. The catalytic triad of PTPS is involved in the deprotonation of the side-chain carbons of substrates.
In addition, Ser 19 of human PTPS may be a substrate for cGMP-dependent protein kinase type II phosphorylation in vivo, which is essential for normal activity of PTPS.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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