Thermo Fisher Scientific eIF4E Polyclonal Antibody
Rabbit polyclonal antibody against human eIF4E. Validated for WB, ICC/IF, and ELISA. Recognizes recombinant eIF4E (aa 1–217). Supplied as liquid, affinity purified, 0.09 mg/mL in PBS with 50% glycerol. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–217 of human eIF4E (NP_0019591) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.09 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804151 |
Product Specific Information
Immunogen sequence:
MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV
Positive Samples: MCF7, 293T
Cellular Location: Cytoplasm, P-body
Target Information
eIF4E is a eukaryotic translation initiation factor involved in several cellular processes, including translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic mRNAs have a 7-methylguanosine cap structure (m7GpppX), to which eIF4E binds, directing ribosomes to initiate translation. It is a 24-kDa polypeptide that exists in free form or as part of the EIF4F complex, which also includes EIF4A (ATPase and RNA helicase) and EIF4G (220-kDa scaffold protein). eIF4E is the rate-limiting component in mRNA–ribosome binding during protein synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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