Thermo Fisher Scientific V5 Epitope Tag Polyclonal Antibody, DyLight 549
V5 epitope tag 인식용 DyLight 549 결합 polyclonal antibody로, Western blot, ICC/IF, ELISA 등에 적합. Rabbit IgG 기반으로 높은 특이성과 감도를 제공. 연구용 단백질 발현 검출 및 위치 분석에 활용 가능.
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Thermo Fisher Scientific V5 Epitope Tag Polyclonal Antibody, DyLight 549
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 95–108 (G-K-P-I-P-N-P-L-L-G-L-D-S-T) of the V protein conjugated to KLH using maleimide |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid; dilute only prior to immediate use.
This affinity purified antibody is directed against the V5 epitope tag and is useful for detecting its presence in overexpressed proteins across various assays.
The antibody recognizes the V5 epitope tag (Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr) fused to either the amino or carboxy termini of targeted proteins in transfected or transformed cells.
Target Information
Epitope tags enable localization of gene products in various cell types, study of protein topology and complexes, identification of associated proteins, and characterization of newly identified or low-abundance proteins when protein-specific antibodies are unavailable.
The V5 epitope tag originates from the P and V proteins of the paramyxovirus simian virus 5 (SV5).
The full V5 tag consists of 14 amino acids (GKPIPNPLLGLDST), though shorter sequences (IPNPLLGLD) are also used.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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