Thermo Fisher Scientific MYC Epitope Tag Polyclonal Antibody, DyLight 549
MYC epitope tag 인식용 Rabbit Polyclonal Antibody로 DyLight 549 형광 표지. WB, ICC/IF, ELISA에 적합하며 높은 민감도 제공. MYC-tag 융합 단백질 검출에 유용. 장기 보관 시 -20°C에서 안정적.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified from rabbit serum immunized with MYC epitope tag peptide (E-Q-K-L-I-S-E-E-D-L) conjugated to KLH. Corresponds to amino acids 410–419 of human c-Myc. |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm (View spectra) |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid repeated freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody specifically recognizes the MYC epitope tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the N- or C-terminus of target proteins in transfected or transformed cells. Useful for detection of MYC-tagged overexpressed proteins in various assays.
Target Information
Myc is a proto-oncogene encoding a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation. It forms a heterodimer with MAX, binding the E-box DNA consensus sequence to regulate transcription. Gene amplification is common in numerous cancers, and translocations are associated with Burkitt lymphoma and multiple myeloma. Translation may initiate from both a non-AUG (CUG) and an AUG start site, producing two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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