Thermo Fisher Scientific 6X His Tag Polyclonal Antibody, DyLight 549
6X His 태그 단백질 검출용 DyLight 549 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA에 적합. 토끼 IgG 기반, 친화 크로마토그래피 정제. 556/570 nm 형광 특성으로 고감도 검출 가능.
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Thermo Fisher Scientific 6X His Tag Polyclonal Antibody, DyLight 549
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the 6X HIS epitope tag (H-H-H-H-H-H) conjugated to KLH using maleimide |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody targets the 6X HIS epitope tag, useful for detecting overexpressed proteins in various assays. It recognizes the His-His-His-His-His-His sequence fused to either N- or C-terminus of target proteins in transfected or transformed cells.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly fused to recombinant proteins to enable purification via immobilized metal affinity chromatography (IMAC).
His-tagged proteins bind strongly to Ni²⁺ ions, allowing efficient purification using Ni²⁺-NTA adsorbents.
His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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