Thermo Fisher Scientific LAMP-2A Polyclonal Antibody (AMC2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

View 42 publications 42 publications

Immunohistochemistry (IHC)

-

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

-

View 19 publications 19 publications

ELISA (ELISA)

0.1-1 µg/mL

-

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Dot blot (DB)

-

View 1 publication 1 publication

Inhibition Assays (IA)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

AMC2

Immunogen

A synthetic peptide of the cytosolic tail of rat LAMP-2A (GLKRHHTGYEQF) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LAMP2 (CD107b),uniProtId:P17046-1,ncbiNodeId:10114,antigenRange:400-411,antigenLength:411,antigenImageFileName:51-2200_LAMP2_CD107b_P17046-1_Rabbit.svg,antigenImageFileNamePDP:51-2200_LAMP2_CD107b_P17046-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533900

Product Specific Information

This antibody was tested for specificity in rat and mouse (it does not cross-react with the human variant) and was demonstrated to be highly selective for LAMP-2A without cross-reacting with LAMP-2B or C. This protein was previously known as lgp96.

In western blotting this antibody was used at 90 ng/mL with 100 µg of rat liver lysosomal protein. This antibody is also able to completely block transport of cytosolic proteins into lysosomes in rat-derived cells.

Target Information

LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon`s disease).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.