
Thermo Fisher Scientific ATP5I Monoclonal Antibody (OTI1G4), TrueMAB
인간 ATP5I 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western Blot 및 IHC(P) 적용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공. 연구용으로만 사용 가능하며 ATP 합성효소 복합체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G4 |
| Immunogen | Full length human recombinant protein of human ATP5I produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Mitochondrial ATP synthases (ATPases) convert electrochemical proton gradient energy into high-energy phosphate bonds.
They consist of two linked complexes:
- F1: Hydrophilic catalytic core
- F0: Membrane-embedded protein channel
The complexes are connected by a central and peripheral stalk.
During catalysis, ATP synthesis in F1 is coupled via rotary motion of the central stalk with proton translocation across the membrane.
ATP5I (also known as ATP synthase subunit E or ATP5K) is a 69-amino-acid protein localized to the inner mitochondrial membrane and forms part of the F0 complex.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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