Thermo Fisher Scientific H3K79me1 Polyclonal Antibody
H3K79me1 단일메틸화 히스톤 H3를 인식하는 Thermo Fisher Scientific의 폴리클로날 항체입니다. WB, ICC/IF, IP, ChIP 등 다양한 응용에 적합하며, Rabbit IgG 기반으로 고순도 친화 크로마토그래피 정제. 다양한 종에 반응하며 -20°C에서 안정적으로 보관 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Immunoprecipitation (IP) | 0.5 µg/mL |
| ChIP assay (ChIP) | 2 µg |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Fruit fly, Human, Mouse, Non-human primate, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 K79-Me1 affinity purified antibody prepared from rabbit serum immunized with a synthetic peptide surrounding Lys79 site of human Histone H3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and refreeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Anti-Histone H3 [Monomethyl Lys79] reacts with human Histone H3.
BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans.
Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is a DNA-binding protein found in eukaryotic chromatin. Together with other core histones, it forms nucleosomes that organize DNA structure.
Histones regulate transcription, DNA repair, replication, and chromosomal stability.
Post-translational modifications (methylation, acetylation, phosphorylation) on the N-terminal tail of H3 influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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