Thermo Fisher Scientific H4K12ac Polyclonal Antibody
H4K12ac 폴리클로날 항체로 인간 히스톤 H4의 Lys12 아세틸화 부위를 인식합니다. Western blot, IHC, ICC, IP, Dot blot 등 다양한 응용에 사용 가능하며, 친화 크로마토그래피로 정제된 고품질 항체입니다. -20°C에서 안정적으로 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:10 |
| Immunoprecipitation (IP) | 1.0 µg/mL |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H4 [ac Lys12] affinity purified antibody prepared from whole rabbit serum immunized with a synthetic acetylated peptide surrounding Lysine 12 of human Histone H4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.69 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
A BLAST analysis suggests cross-reactivity with Alfalfa based on 100% sequence homology.
Cross-reactivity with Histone H4 [ac Lys12] from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction.
This gene is intronless, encodes a histone H4 family member, and is part of the large histone gene cluster on chromosome 6. Transcripts lack polyA tails but contain a palindromic termination element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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