Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), FITC, eBioscience

Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 711 / 786
• eFluor 450
• Functional Grade
• NovaFluor Blue 510 / 610-30S
• NovaFluor Red 685 / 710
• NovaFluor Yellow 570 / 590 / 660
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage.
CD24 is variably glycosylated, resulting in heterogeneity of molecular mass among different cell lineages. Antibodies to CD24 exhibit subtle differences in staining levels on lymphocyte populations.
Expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes and thymocytes.
Use ≤ 0.5 µg/test (defined as antibody amount for staining in 100 µL volume). Cell number: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B-cell in bone marrow to mature surface Ig-positive B-cells. Plasma cell expression is low or negative.
CD24 is expressed on most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may play a role in regulation of B-cell proliferation, maturation, and autoimmunity control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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