
Thermo Fisher Scientific MGMT Monoclonal Antibody (OTI2E2), TrueMAB
MGMT 단백질을 인식하는 Mouse 모노클론 항체로 Western blot 및 IHC(P) 검증 완료. Human 시료 반응성. 동결건조 형태로 제공되며 재구성 후 약 1 mg/mL 농도. DNA 손상 복구 연구 및 알킬화제 내성 메커니즘 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E2 |
| Immunogen | Full length human recombinant protein of human MGMT produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
O6-Methylguanine-DNA Methyltransferase (MGMT) is a DNA repair protein involved in tumor cell resistance to alkylating chemotherapeutic agents. It protects normal cells from genotoxic and carcinogenic effects of DNA alkylation by removing alkyl groups from the O6 position of guanine. MGMT expression varies by cell type, tissue, species, and tumor characteristics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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