Thermo Fisher Scientific TYMS Monoclonal Antibody (OTI3F1), TrueMAB
TYMS 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot과 IHC(P) 실험에 최적화됨. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제, 동결건조 형태로 안정적 보관 가능. DNA 생합성 연구 및 항암제 반응 분석에 활용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F1 |
| Immunogen | Full length human recombinant protein of human TYMS produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), essential for DNA biosynthesis.
TS is a key target of fluoropyrimidines, a class of antineoplastic drugs used for treating solid tumors.
5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP, which inactivates TS by forming a ternary covalent complex with the folate cofactor 5,10-methylenetetrahydrofolate.
Expression of TS protein correlates with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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