Thermo Fisher Scientific CD357 (AITR/GITR) Monoclonal Antibody (eBioAITR), PE-Cyanine7, eBioscience

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested dilution: Not specified
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested dilution: 5 µL (1 µg)/test
• Publications: View 4 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• eFluor 450
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody reacts with human AITR (Activation Inducible TNFR family member), a ~25 kDa member of the TNFR superfamily. AITR mRNA is detected in lymph node, peripheral blood leukocytes, and weakly in spleen. Expression is upregulated within 24 hours after PMA/ionomycin or PHA stimulation of PBMCs. At the protein level, AITR is expressed by a small population of activated PBMCs. AITR associates with TRAF1, TRAF2, and TRAF3, inducing NF-κB activation via TRAF2. TL6 (AITRL) has been reported as its ligand, mediating cross-talk between T lymphocytes and endothelial cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometry using stimulated normal human peripheral blood cells. Use 5 µL (1.0 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).

Light Sensitivity: Protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark. Clone-specific performance should be confirmed empirically.

Excitation: 488–561 nm
Emission: 775 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also known as GITR or AITR, is encoded by the TNFRSF18 gene located at 1p36.33. This receptor shows increased expression upon T-cell activation and plays a key role in immunological self-tolerance maintained by CD25⁺CD4⁺ regulatory T cells. Knockout studies in mice indicate its role in regulating CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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