Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), PE-Cyanine7, eBioscience

Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), PE-Cyanine7, eBioscience™

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.125 µg/test
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Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• NovaFluor Red 710
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of mature B cells in bone marrow and peripheral tissues.
Fluorochrome-conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse spleen cells. Recommended ≤0.125 µg/test (100 µL final volume). Optimize empirically for best performance.

Light Sensitivity: Tandem dye is photo-sensitive; protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission: 488–561 nm / 775 nm (Blue, Green, Yellow-Green lasers).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody produced during an immune response and is the largest immunoglobulin in serum. Composed of five heavy and five light chains, it plays a key role in complement activation and antigen agglutination.
Widely used in research and diagnostics (ELISA, Western blotting, immunohistochemistry).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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