Thermo Fisher Scientific Human IL-13 Recombinant Protein
인간 IL-13 재조합 단백질로 면역 및 염증 반응 연구에 활용. E. coli 발현, 순도 95% 이상, 엔도톡신 <0.1 ng/µg. 인간 TF-1 세포 증식 활성 검증. 동결건조 형태로 제공되며, 재구성 후 -20°C 이하 보관 권장.
- 카탈로그번호
- PHC013x (3개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human IL-13 Recombinant Protein
Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Functional Assay | Assay-dependent | |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD LLLHLKKLFR EGRFN |
| Molecular Weight | 12.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 1.0 ng/mL; determined by dose-dependent proliferation of human TF-1 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Before opening, briefly centrifuge the vial to collect contents at the bottom. Reconstitute lyophilized hIL-13 in sterile, deionized water to a concentration of 0.1–1.0 mg/mL for full activity. Prepare aliquots and store at ≤ -20°C. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage:
Store lyophilized hIL-13 at 2–8°C, preferably desiccated. After reconstitution, store at ≤ -20°C (not in frost-free freezer). Minimize freeze-thaw cycles.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses by down-regulating macrophage activity, inhibiting pro-inflammatory cytokine and chemokine production, and contributing to allergen-induced asthma independent of IgE and eosinophils. IL-13 also influences B cell maturation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching. Structurally similar to IL-3, IL-4, IL-5, and GM-CSF, the IL-13 gene is located on chromosome 5q near IL4 within a cytokine gene cluster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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