Thermo Fisher Scientific Human IL-18 Recombinant Protein

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human IL-18, amino acids 37-193

Molecular weight

18 kDa

Class

Recombinant

Type

Protein

Purity

>90% by SDS-PAGE

Endotoxin concentration

<0.1 ng/µg

Activity

Determined by the dose-dependent production of IFN-gamma by human KG-1 myelomonocyte cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS with 0.1% BSA, 1% sucrose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Reconstitution: We recommend the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute with 250 µL ice cold, sterile, distilled water on ice.

Storage: Store this lyophilized preparation at -20°C, preferably desiccated. Upon reconstitution, the protein may be stored at 2°C to 8°C for up to one week. For long term storage of the reconstituted protein, apportion into working aliquots and store at -80°C. Avoid repeated freeze/thaw cycles.

Target Information

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.